What is CrispR?

What is CrispR?

  • CRISPR stands for “clustered regularly interspaced short palindromic repeats”.
  • It is a method of DNA editing more efficient than ones available.
  • CRISPR uses an enzyme called Cas9 to cut strands of DNA at precisely targeted locations.
  • This allows one to insert new genetic material into the gap.
  • CRISPR promises to revolutionize gene editing in organisms, by modifying inherited genes to spread a trait throughout a population.
  • CrispR is a replica of the natural process when a virus infects a bacterium.
    • When a virus gets in to bacterial cell wall, it becomes integrated into the bacteria’s genome.
    • From there, the viral DNA will replicate and become translated/transcribed into proteins which will eventually form a newly synthesized virus.
    • In order to defend against viral infection, bacterium evolved the CRISPR/Cas9 system adaptive immune system.
    • Within this system, ‘bacterial’ CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) spacer sequences are transcribed into RNA which guides a Cas9 endonuclease to matching regions of ‘viral’ DNA within the hosts’ genome.
    • The Cas9 endonuclease then unwinds the host cell DNA and cleaves/removes the infectious viral DNA from the bacterium.

How do Genes work?

  • Every cell in an organism contains all of the information needed to manufacture every protein in its body.
  • The genes in strands of DNA are a storehouse of information, an instruction book.
  • The genes that an organism carries for a particular trait is its genotype and the physical manifestation of the instructions are the organism’s phenotype.
  • Further a gene (a sequence of bases in a section of DNA) affect the phenotype in two main steps.
  • Transcription, in which a copy of a gene’s base sequence is made, and
  • Translation, in which that copy is used to direct the production of a protein.
  • In transcription, the gene’s base sequence or code is copied into a middleman molecule called messenger RNA (mRNA).
  • In translation, the mRNA moves out of the nucleus and into the cytoplasm of the cell, where the messages encoded in the mRNA molecules are used to build proteins.
Section : Science & Tech

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